abstract：:NADH : FMN oxidoreductase and bacterial luciferase have been efficiently coimmobilized onto Sepharose 4B. This luminescent immobilized enzyme system can be used to assay NADH. The assay is rapid and sensitive with a lower limit of detection of 0.2 pmol/assay tube. The intra-assay precision was 3.5% at 2 × 10(-5) M and...

journal_title：Applied biochemistry and biotechnology

authors： Wienhausen GK,Kricka LJ,Hinkley JE,Deluca M

更新日期：1982-11-01 00:00:00

abstract：:Alkaline phosphatase from hog intestine was immobilized to controlled-pore glass under various conditions. The specific activity of the enzyme was not diminished by immobilization. The influence of temperature and pH on the behavior and the stability of the immobilized enzyme preparations is discussed and compared to ...

journal_title：Applied biochemistry and biotechnology

authors： Pittner F

更新日期：1982-05-01 00:00:00

abstract：CONCLUSION:Immobilization of the thermophilic lactase causes a decrease in the formation of intermediary products. This means that partially hydrolyzed lactose syrups contain less of these intermediary products when the immobilized thermophilic lactase is used in the hydrolysis process than when a soluble lactase is us...

journal_title：Applied biochemistry and biotechnology

authors： Rugh S

更新日期：1982-01-01 00:00:00

abstract：:A model system for evaluating macroporous supports containing immobilized whole cells in affinity Chromatographic applications is described. Whole cells were immobilized in a polyacrylamide network in a two-step polymerization process. The affinity system discussed consists of immobilized cells ofSaccharomyces cervisi...

journal_title：Applied biochemistry and biotechnology

authors： Ramstorp M,Mattiasson B

更新日期：1982-01-01 00:00:00

abstract：:A metal oxide semiconductor sensitive to hydrogen was used to monitor H2 production from immobilizedClostridium acetobutylicum cells and from immobilized hydrogenase on a continuous basis. ...

journal_title：Applied biochemistry and biotechnology

authors： Winqvist F,Danielsson B,Lundström I,Mosbach K

更新日期：1982-01-01 00:00:00

abstract：:The use of immobilized enzymes has opened the possibility of large scale utilization of NAD(+)-linked dehydrogenases, but the applications of this technique were limited by the necessity of providing the large amounts of NAD(+) required by its stoichiometric consumption in the reaction. After immobilization of alcohol...

journal_title：Applied biochemistry and biotechnology

authors： Burstein C,Ounissi H,Legoy MD,Gellf G,Thomas D

更新日期：1981-12-01 00:00:00

abstract：:A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjug...

journal_title：Applied biochemistry and biotechnology

authors： Tadolini B,Hakim G

更新日期：1981-09-01 00:00:00